This reagent kit is for quantitative estimantion of Amylase activity in serum or plasma.

Clinical Significance

Amylase assay performed in cases of detecting pseudocyst ascites and pleural effusion following acute pancreatitis. Increased level of amylase may also be realted to disorders like biliary tract, diabetic ketoacidosis, severe glomerular/dysfuction, salivary gland disorders and ruptured ectopic pregnance. Incase of acute pancreatitis, serum level of Amylase is elevated after 4 hours of onset of pain and reaches peak at 24 hours and remains elevated for 3-7 days.

Specimen Collection And Storage

Fresh, clear, and fasting unhemolysed serum is preferred. Anticoagulants like oxalates, citrates, and EDTA should be avoided.