One step immunochromatographic assay for qualitative detection of infection with Plasmodium falciparum and Plasmodium vivax causing malaria
Principle
Sandwich Immunoassay
Technique
Lateral flow(ICT)
Detection
Histidine rich protein 2 specific for Plasmodium falciparum (P-HRP-2) Plasmodium lactate dehydrogenase common to all Plasmodium species (Pv – pLDH)
Capture
Pf Region -Histidine rich protein 2 specific for Plasmodium falciparum (P-HRP-2)
Pv Region – Plasmodium lactate dehydrogenase common to all human Plasmodium species (Pv – pLDH)
Conjugate
Antibodies specific to P-HRP-2 and Pv – pLDH coupled with colloidal gold
